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cotransporter While stem cell based therapies
2018-11-12
While stem cell-based therapies hold great potential for the treatment of a wide array of medical conditions they are so novel that product characterization is particularly challenging. Despite considerable progress, the molecular regulatory mechanisms of self-renewal and lineage specification in th
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Attempts to expand olfactory stem cells
2018-11-12
Attempts to expand olfactory stem ldv according to the free-floating cell aggregate central nervous system (CNS) neurosphere culture method (Reynolds and Weiss, 1992) have been described (Barraud et al., 2007; Sicard et al., 1998; Carter et al., 2004). Olfactory spheres (OSs), clusters of cells gene
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adrenergic receptors The following is the supplementary data
2018-11-12
The following is the supplementary data related to this article. Acknowledgments This work was partially supported by a grant (NSC98-2311-B-003-MY3 and NSC101-2311-B-003-005) to Y. Lin from the National Science Council, Taiwan. We are grateful for the help from the Molecular Imaging Core Facilit
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Recently our group elucidated the role of the
2018-11-12
Recently, our group elucidated the role of the endothelium and secretion of angiocrine factors in the expansion of mouse and human HSCs (Butler et al., 2010a, 2010b; Kobayashi et al., 2010). We developed a model of endothelial melk pathway with autonomous Akt-activation (HUVECs-E4ORF1, referred to
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Genetically engineered stem cells may
2018-11-12
Genetically engineered stem cells may have an advantage for gene therapy due to their inherent migration to tumor sites (Kang et al., 2012a; Kang et al., 2012b; Kang et al., 2012d). In this study, we employed HB1.F3 human neural stem cells (hNSCs) obtained from 15week fetal telencephalon and immorta
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Germ and somatic cells or pluripotent and differentiated cel
2018-11-12
Germ and somatic cells, or pluripotent and differentiated PR619 can be distinguished on the basis of their epigenetic states (Boland et al., 2009; Okita et al., 2007; Takahashi and Yamanaka, 2006; Yamanaka, 2012). We previously demonstrated that epigenetic reprogramming following somatic cell nucle
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Immunocytochemistry Cells were fixed at room temperature wit
2018-11-12
Immunocytochemistry. Cells were fixed at room temperature with 4% paraformaldehyde for 10min. Non-specific proteins were blocked by ultra V block (Thermo). The cells were then treated with primary gnrh antagonist overnight at 4°C. Primary antibodies were against TRA-1-60 (1:500, MA1-023, Thermo Fis
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br Materials and methods br
2018-11-09
Materials and methods Results Discussion Several in vivo and in vitro studies indicate that OPCs have substantially different properties according to their chronological age. For example, human fetal OPCs from different gestational stages exhibit differences in the myelination profile (Cui
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The most commonly used trophoblast markers
2018-11-09
The most commonly used “trophoblast” markers reported in the literature are cytokeratin 7 (KRT7), HLA-G, and human chorionic gonadotropin (hCG), but these are either not specific to all trophoblast p38 pathway or are expressed in other cell types. Several of the transcription factors (TF) that defi
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Only the bands connected to the optical
2018-11-09
Only the α2 bands connected to the optical electron transitions from the EO quasilocal states, located deep in the valence band, to spreading states near its upper edge do not have any analogies in literature. It should be noted that these bands are present only in the α(hν) spectra of the oxygen-im
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Harman and Stewardson mentioned that only a few
2018-11-09
Harman and Stewardson (2005) mentioned that only a few published studies explicitly described the link between environmental flow targets and dam operating rules. To resolve the river management-driven issues and bridge the gap between a simple minimum stream flow target and the great complexity of
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A striking challenge in studying Alzheimer s disease is the
2018-11-09
A striking challenge in studying Alzheimer\'s disease is the use of appropriate animal models. Since Aβ peptides derived from murine APP do not lead to neurodegeneration, almost all animal studies are based on humanized mice overexpressing human APP. Moreover, the mutated APP variant inserted in the
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between We did not find any difference in
2018-11-09
We did not find any difference in the percent of NBC infected with low or high MOI by IFA or RT-PCR. ZIKV could potentially use DC-SIGN and phosphatidylserine receptors, such as T-cell immunoglobulin and mucin domain (TIM) and TYRO3, AXL, and MER (TAM; Hamel et al., 2015; Meertens et al., 2012), or
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Efforts to reduce life cycle CO
2018-11-09
Efforts to reduce life ll-37 CO2 emissions of fuel-efficient cookstoves must focus on reductions in use-phase emissions even if those reductions come at the cost of dramatically increased emissions in other life cycle phases. Take the example of an exceptionally energy-intensive material or process
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notch signaling br Attributing public health burden of youth
2018-11-09
Attributing public health burden of youth behavior to neurodevelopmental patterns Previous reviews have addressed the possibility that the maturation of cognitive control neurocircuitry in adolescence is already sufficient for rational decision-making (Epstein, 2007), at least in explicit, delibe
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